Basic Requirements for Plant Tissue Culture Laboratory

Plant tissue cultures should be incubated under well-controlled conditions of temperature, lighting, photoperiod, humidity and air circulation. Incubation grow rooms, commercially available incubator cabinets, large plant growth chambers and walk-in environmental rooms meet these requirements. Tap water is rich in unwanted salts, particles (silt, organic matter, etc.) and microorganisms (algae, fungi and bacteria). These can disrupt environments and interfere with crop growth or contaminate the entire crop, resulting in massive losses Tissue culture technology has facilitated the cultivation of plants of interest in an artificial environment. Over the years, small propagators or large commercial enterprises have adapted the technique to grow and develop. However, setting up a tissue culture lab isn`t a breeze for everyone and it`s also a bit expensive. This chapter explains the laboratory requirements and general techniques for the plant tissue culture laboratory. A standard tissue culture laboratory should provide facilities for: (1) washing and storage of laboratory equipment; (2) the preparation, sterilization and storage of culture media; (3) aseptic handling of plant material; (4) the conservation of crops under controlled conditions, (5) the observation of crops; and (6) acclimatization of plants developed in vitro. Different types of containers have been used to grow plant matter.

The incubation space of crops maintained for controlled temperature, light and humidity is discussed in this chapter. Precautions are mandatory to prevent the introduction of microorganisms into the culture vessels. Moving material indoors (sterilized medium, instruments, water) and outdoors (glassware and other things for washing and sterilization, plants made in tissue culture for hardening) clean areas by double-door hatches should help maintain higher asepsis in the clean area. Tissue culture plants are very useful because they are uniform and you can get high-quality plants in a relatively short time. Various companies can benefit from the cultivation of plant tissues in innovative ways. And if you want to learn more about how growing plant tissue can help you make a profit, take a look at our blog on „8 Ways Growing Plant Tissue Makes a Profit.“ Facilities for the cultivation of basic plant tissues comprising any type of in vitro process shall include certain essential elements: (d) grow rooms or incubators for the preservation of crops under controlled conditions of temperature, light and humidity; To design your lab, there are a few things you need depending on the purpose and activity level. The main objective of tissue culture is on the „aseptic state“, which must be maintained at all levels of culture processes. A list of basic requirements for tissue culture laboratories is explained here. Wherever you want to design your lab, these are four areas that you need to assign to the purposes explained below. If you are planning to build a tissue culture facility for research or commercial purposes at small to large production levels, you can always contact our wonderful team for advice and guidance! We specialize in the design and implementation of laboratories for plant tissue cultures and seed selection. Also for specific laboratory needs, questions or additional support, we are here to help! b) The isolated part of the plant must be given an appropriate environment that helps to divide the cell and maintain the expression of internal potential. Let`s dive a little deeper into these areas of a plant tissue culture lab! You will need this area to wash explants or other plant matter.

You`ll also need it to clean all your beakers, cylinders, and bottles that you use to prepare racks or measure ingredients. This area consists of large sinks and drainage panels. This area also has access to distilled water with space to dry. Thus, it facilitates pre-cleaning to kill microorganisms present on the surface. Gyro agitators are used for suspended crops. Air conditioners and heaters are used to keep the temperature around 25 ± 2 °C, and humidity is maintained by uniform forced ventilation. Lighting is also done in measured quantities, i.e. 40-200 fc (foot candle). The development of tissue culture protocols for different plant species and varieties requires extensive trial and error experiments. They experiment with recipes of culture media for different stages of growth and also with different growing conditions in the growing/growing area. Therefore, in this area, you need effective recording practices for different types of tissue culture experiments. Therefore, it`s a great idea to use a desktop computer to write reports and analyze data! When a person is ready to build a lab, they need little practice and an understanding of the basics to get started.

This article is for that person only; We have found cheaper alternatives for small businesses or an amateur. This article also discusses the approximate cost of setting up your lab, safety measures, and tips for setting up your lab. The grow room should have a uniform humidity of 20-80%, and the air conditions installed in this room should have controllers to adjust the temperature and humidity accordingly. Previously, this technique was used either by researchers or by large companies to obtain secondary compounds. In recent years, many amateurs, florists, gardeners and a few other producers have shown interest in this technique either for the purpose of breeding, collecting plants that are threatened or difficult to grow using conventional methods, or for the mass production of horticultural plants (including fruits, vegetables and ornamental plants). First, let`s talk about a basic configuration of a commercial lab. Cultural spaces are built with adequate air conditioning; Perforated shelves (Fig. 16.2D) to support culture vessels, equipped with fluorescent tubes with a timing device to maintain the photoperiod, black curtains may be used to maintain total darkness. After transferring explants and shoots to the environments, you need to store them in ideal conditions for effective growth and development. This means that you need to provide controlled temperature, humidity, air circulation, light quality and light duration. It should be noted that an automated climate and air filtration system is best suited to regulate these conditions.

You need this area for the preparation of recipes of culture media and for the storage of chemicals and glassware. You can use a large area for media preparation with benches. This area also has hot plates, pH meters, scales and water baths. You can also choose a separate area for the media dosing unit connected to your media preparation or autoclave in the processing area. The most important thing we need to know is the equipment we need for the preparation of media, namely: tissue culture techniques can be successfully carried out only in a very clean laboratory with a dry atmosphere and protection from airborne microorganisms. For this purpose, a sterile, dust-free room/cabinet is required for routine transfer and handling work. For each tissue culture laboratory, there are certain basic requirements or facilities that you need to install for effective operation and experimentation. These are: There should also be space to install drying cabinets, washing machines, plastic or steel buckets for soaking laboratory equipment, acid or detergent baths, pipette washing machines, dryers and cleaning brushes. For the storage of washed and dried laboratory tableware, the laboratory must be equipped with dust-tight storage cabinets or cabinets. These environmental factors are important for plant cells to develop at later stages and differentiate into healthy shoots and roots. These specific conditions differ according to plant species.

Therefore, you should be careful when providing the necessary growing environment for the optimal development of tissue culture plants. Stories of selected PCT products are also featured on our website. Not to mention, some treats might find a way to access your home. Share your suggestions and story with me at anjali@plantcelltechnology.com Many of you may be curious about how to set up your own tissue culture lab. What are the requirements? What equipment do you need? Where should you be careful when planning a setup? In this article, we will briefly try to solve this puzzle! To set up your plant tissue culture laboratory, it is important that you choose the location carefully. The sterile environment of a tissue culture laboratory is susceptible to contamination by ventilation slots. You should try not to build it near a plant that deals with microorganisms (bacteria, fungi, etc.). You should also try that it should not be near other laboratories that deal with insects. Another important point is that the laboratory must be away from the storage places where seeds and old plant material are stored.

However, if you can`t avoid them, you`ll need to take extra precautions to keep the lab free of contaminants.

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